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Other effects of mitragynine are local anesthesia and central nervous system depression. Heavy use can result in a prolonged sleep. Lucky Kratom Extract Liquid bali) Kratom extract can be mixed with any liquid (hot water or a milk shake for example).

To assess the effect of red vein sumatra kratom MSE on cell proliferation and kratom x15 effects viability the Trypan Blue exclusion assay was performed. This assay could be used with much higher concentrations of MSE and showed dose and time-dependency in cell proliferation and

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viability. The individual results for each type of cell line are as follow: a. HepG2 cells Within 24 hr there was a clear dose-dependent loss of cell proliferation compared to the vehicle-treated Lucky Kratom Extract Liquid control (Fig. The effect became pronounced at doses higher than 1. With vehicle-treated control there were very few cell dead cells irrespective of the time in culture. There was a distinct threshold for cytotoxicity at doses higher than 11.

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Examples of chemicals in this class are methylnitrosourea (MNU) methylmethanesulfonate (MMS) ethyl methanesulfonate (EMS) etc. Aspergillus flavus and A. Friedberg et al 2006).

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However the trend towards toxicity was only seen at doses of MSE in excess of 0. Similarly no statistically significant toxicity was observed on HepG2 proliferation over this dose range (Fig. A complication found using this assay was that high concentrations of MSE interfered with the assay measurement. Therefore an alternative assay (Trypan blue exclusion) was used to examine the effect of higher concentrations of MSE on cell toxicity. Effect of MSE on cytotoxicity (A) and proliferation (B) of HepG2 cells after 24 hr of kratom joint treatment.