MSE suggested that 24 hr was the time point at which kratom capsules cheap the changes began to be noted. On reflection the interpretation of these latter experiments would have been improved by comparison to control groups for each time points. Tony’s Smoke Shop Kratom Holly Tony’s Smoke Shop Kratom Holly Grove Grove
Subsequently the cell cycle distribution of SH-SY5Y cells treated with MSE and MIT was examined as they were the most sensitive cells examined to date.
M MIT where cells accumulated at G1 phase and the population shifted to the right side of the scale. This phenomenon implies that the treated cells have taken up more PI dye thus leading to a shift to the right. Due to the amount of MIT compound available repetition of this experiment was not possible. Effects of MSE on the cell cycle distribution of SH-SY5Ycells after 48 hr of treatment. MSE on the cell cycle distribution of SH-SY5Y cells at different time points (4 8 24 48 72 and 96 hr treatment). Tony’s Smoke Shop Kratom Holly Grove Indicates only one experimental result.
Based on the literature it was well known that p53 has the ability to induce G1 arrest and its target gene p21 facilitates the arrest (Ko and Prives 1996) by inhibiting the function of CDKs (Gu et al 1993; Harper et
al 1993). Therefore the role of p53 and p21 in MSE and MIT induced toxicity were examined. However
in the present studies the cell cycle Tony’s Smoke Shop Kratom Holly Grove arrest noted appeared to be independent of induction of p53 and p21. The loss of the protein was can kratom be drug tested strongly dose-dependant as there was a time dependant induction of p53 expression observed in the control and lower dose groups indicating a normal p53 expression response in this cell line.
In the present study it is suggested that the toxicity effects seen for MSE were predominantly due to MIT as shown by similar IC50 values for MIT and MSE treated SH-SY5Y cells. The role of metabolism was also assessed in which the toxicity of MSE treated SH-SY5Y cells was found to increase 10-fold when the metabolic activation system post mitochondrial rat liver S9 induced by Arochlor 1254 was added to the treatment. However contradictory results were noted when metabolically competent MCL-5 cells appeared to detoxify MSE rather than side effects of kratom use activate it.